Leiden Muscular Dystrophy pages - Protocols^©

Figures; myoD-induced myo-differentiation

(Judith van Deutekom/Anneke Janson; last modified on March 25, 2000)

Figures

1. The method; myoD-induced myo-differentiation of non-muscle cells
2. Adenovirus mediated myo-differentiation
3. Variable adenoviral infection of chorion villi cells
4. Immunohistochemical analysis; desmin / myosin double-staining
5. Immunohistochemical analysis; myosin / dystrophin double-staining

Information

Figure 1

Figure 2

Figure 3

Figure 3
Adenoviral infection of different chorion villi cell cultures. Ten different chorion villi cell cultures were infected with an adenoviral vector carrying the LacZ gene (Ad5-LacZ), at increasing MOIs. The results show the high variability in adenoviral transduction efficiencies in the individual cell cultures, determined as the percentage of beta-galactosidase positive cells.

Figure 4

Note the appearance of multi-nucleated cells, indicating successful differentiation towards muscle-like cells.

Figure 5

Note that the myosin staining is througout the cell, while dystrophin staining is more in the cell membrane.

Information

Dr. Judith C.T. van Deutekom
        Department of Human and Clinical Genetics, Leiden University Medical Center
        E-mail: deutekom@lumc.nl / Tel: +31 - 71 - 527 6080

Dr. Johan T. den Dunnen
        Department of Human and Clinical Genetics, Leiden University Medical Center
        E-mail: ddunnen@lumc.nl / Tel: +31 - 71 - 527 6105

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