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Eemrin (EMD) primers

(last modified December 29, 2004)


Contents



DNA-primers

Exon Position Length
(in bp)
Forward / reverse primer Name Reference
1 - 6 -226 - 841 2006 CTCCCGCGGTTAGGTCCCGCCC /
CACCCCCACTGCTAAGGCAGTCAGC
PLf / PMr Bione (1995)
1 -176 GCGGCCGTGACGCGACAACG PFf Bione (1995)
1 -20   CGCCTGAGCCCGCACCCGC PCf Bione (1995)
2 103   CGTAAAGCCTACGAGTTGATCg P6r Bione (1995)
intron 2 187+37   gggaggatggggtcgcgagg P13f Bione (1995)
intron 2 188-54   ttctccagtgccgctctcgac P12r Bione (1995)
intron 3 266-77   tgggattcagattagggccatcag P14f Bione (1995)
intron 3 266-93   cagtttctccctcctaaggctgc PGr Bione (1995)
intron 4 399+96   ccaccatttgtacccagtgcc P15r Bione (1995)
intron 4 400-187   gcatgagcacaagtggcaaggc P16f Bione (1995)
intron 5 449+19   cagggacgggctggttctggg P17f Bione (1995)
6 500   TAGTGCGTGATGCTCTGGTAGG P18r Bione (1995)
6 642    GGCTGGGCCAGGATCGCCAG P8f Bione (1995)
6 728   TAAATGAAGAAGAGGACGATCAC P4r Bione (1995)

Legend:
Exonic sequences are in upper case, intronic and gene flanking sequences in lower case and added primer tails in italics. Location: location of primer (numbering of exons is according to Bione et al. [1995]).Position: position of primer (numbering according to the coding DNA Reference Sequence). Length (in bp): length of PCR-product in basepairs. Forward / reverse primer: sequence of forward and reverse primer. Name: name of the primers. Reference: publication describing the primer(s).


RNA-primers


Exon Position Length
(in bp)
Forward / reverse primer Name Reference
1 - 6 -226 - 841 2006 CTCCCGCGGTTAGGTCCCGCCC /
CACCCCCACTGCTAAGGCAGTCAGC
PLf / PMr Bione (1995)

Legend:
Exonic sequences are in upper case, intronic and gene flanking sequences in lower case and added primer tails in italics. Location: location of primer (numbering of exons is according to Bione et al. [1995]).Position: position of primer (numbering according to the coding DNA Reference Sequence). Length (in bp): length of PCR-product in basepairs. Forward / reverse primer: sequence of forward and reverse primer. Name: name of the primers. Reference: publication describing the primer(s).



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