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DMD (dystrophin) cDNA probes

(last modified on October 14, 2004)


The probes are used for hybridizations to genomic DNA and can be used to identify the exact number of deleted or duplicated exons.


cDNA probe synoniem exons bordering RE-sites vector insert Reference ATCC
cDNA9-7 cDNA(1-2a) 1-11 (BamHI)/(HindIII) pBluescribe 1.5 kb Koenig (1987) 57666, 57667
cDNA.XJ10 cDNA(1-2) 1-15 (EcoRI)/(EcoRI) pBluescript 2.0 kb Worton  
Cf27 cDNA(1a-3a) (3)-(20) (EcoRI)/EcoRI lambda gt11 2.2 kb Forrest (1988)  
cDNA30-2 cDNA(2b-3a) 11-(20) (EcoRI)/EcoRI pBluescribe 1.2 kb Koenig (1987) 57668, 57669
Cf16   11-16 .../...   0.6 kb GenBank M22819  
Ca1B         0.9 kb Cross (1987)  
cDNA30-1 cDNA(3b-5a) 20-31 EcoRI/(EcoRI) pBluescribe 2.0 kb Koenig (1987) 57670, 57671
Ca1A   (12)-25 (EcoRI)/...   1.9 kb Cross (1987), GenBank M22818  
Cf23 cDNA(3b-7) 20-(47) EcoRI/EcoRI lambda gt11 4.3 kb Forrest (1988)  
Cf23b cDNA(3b-6a) 20-37 EcoRI/PstI lambda gt11 2.8 kb Forrest (1988)  
cDNA47-4 cDNA(5b-7) 31-(47) (EcoRI)/EcoRI pBluescript KS 2.5 kb Koenig (1987) 57672, 57673
Cf23a cDNA(6b-7) 37-(47) PstI/EcoRI lambda gt11 1.5 kb Forrest (1988)  
Cf56b cDNA(6b-7) (38)-(47) (EcoRI)/EcoRI lambda gt11 1.3 kb Forrest (1988)  
  cDNA(7b-8) 44-(53) (BglII)/EcoRI pKUN1 1.37 kb DB: Leiden  
cDNA44-1 cDNA(8) 47-(53) EcoRI/EcoRI pBluescript KS 0.88 kb Koenig (1987) 57674, 57675
Cf56a cDNA(8) 47-(53) EcoRI/EcoRI lambda gt11 0.88 kb Forrest (1988)  
cDNA63-1 cDNA(9-14) 53-79 EcoRI/(EcoRI) pBluescript KS 6.10 kb Koenig (1987) 57676, 57677
Cf115 cDNA(9-13a) 53-79 EcoRI/(EcoRI) lambda gt11   Forrest (1988)  
Cf77a cDNA(9-..) 53-(..) EcoRI/(EcoRI) lambda gt11 1.3 kb Forrest (1988)  
63-1/1 cDNA(9) 53-59 EcoRI/BamHI isolated fragment of cDNA63-1 1.11 kb Den Dunnen (1989)  
  cDNA(9a)   EcoRI/Pst isolated from cDNA63-1 EcoRI/HincII fragment 0.3 kb Covone (1991)  
  cDNA(9-10)   EcoRI/HincII isolated fragment of cDNA63-1  kb Covone (1991)  
63-1/2 cDNA(10) 59-(66) BamHI/BamHI isolated fragment of cDNA63-1 0.71 kb Den Dunnen (1989)  
63-1/3 cDNA(10b-11a) 66-74 BamHI/BglII isolated fragment of cDNA63-1 0.90 kb Den Dunnen (1989)  
  cDNA(11)   EcoRI/HincII isolated fragment of cDNA63-1  kb Covone (1991)  
63-1/4 cDNA(11b) (74)-(77) BglII/BglII isolated fragment of cDNA63-1 0.40 kb Den Dunnen (1989)  
63-1/HcE cDNA(12a) 76-79 EcoRI/BamHI isolated fragment of cDNA63-1 0.6 kb Den Dunnen (1989)  
  cDNA(12-14)   EcoRI/HincII isolated fragment of cDNA63-1  kb Covone (1991)  
63-1/e cDNA(13-14) 79 XbaI/(SalI) isolated fragment of cDNA63-1 2.0 kb Den Dunnen (1989)  

Legend:
cDNA probe: name of cDNA probe. Synoniem: alternative name of cDNA probe. Exons: exons covered by the probe, bracketed if the probe spans the exon only partially. Bordering RE-sites: sites flanking the insert, bracketed if the site is lost upon cloning. Vector: vector used for cloning. Insert: insert size in kilo basepairs. Reference: publication describing the cDNA probe. ATCC: ATCC reference number.



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