From: "dr. Johan T. den Dunnen"To: ddunnen@lumc.nl Subject: acquiring a rat EST library Date-Sent: 02 February 2000 23:18 +0100
Dear ArrayNL platform members, Please find below an urgent request to particiapte in acquiring a rat cDNA/EST clone collection for expression array studies. One of the goals of our platform was to promote such type of efforts; now we have the chance to see if our platform is able to achieve something by collaborative efforts. Please, when interested, do not sit down and wait for others to become active but reply as soon as possible. It would not be the first initiative which raises a large interest but still fades out because the interests are not made known. yours sincerely, Johan den Dunnen Dept. Human and Clinical Genetics Leiden University Medical Center ======================================================================== Dear ArrayNL platform members, In addition to the available set of 40,000 human EST clones the Leiden Genome Technology Center (LGTC, Leiden University Medical Center) wishes to acquire other clone sets. High on the list is a set of 17,000 rat EST clones from Research Genetics. Since this particular collection of clones is of potential interest to numerous research groups in the Netherlands we are looking for those that are willing to participate in purchasing this library. In the end, purchasing the entire library is much cheaper than ordering individual clones. The cost of the library is approximately $ 100,000. Individual clones are $45 for the first clone in each order and $24 for each additional clone. This means that about 4000 individual rat EST clones need to be ordered to cover the cost of the entire library, a number that should be easily reached combining the requests from the Netherlands. Right now the Division of Medical Pharmacology of the Leiden University Medical Center (Dr. Erno Vreugdenhil / Dr. Nicole Datson) has a request for over 100 rat cDNA-clones. Instead of ordering the clones individually this money could be put towards obtaining the full librabry, making it accessible to a larger research community. In collaboration with the LGTC the Division of Medical Pharmacology is willing to make a significant contribution in obtaining the library. The LGTC is willing to store it and to handle clone requests. The LGTC will handle clone requests from groups which have participated in purchasing the library free of charge. Our urgent request is for those who have an interest in the rat EST set of clones and are willing to contribute significant finances in it's purchase, to contact Nicole Datson (datson_n@lacdr.leidenuniv.nl) as soon as possible. Yours sincerely, Nicole Datson Division of Medical Pharmacology Leiden/Amsterdam Center for Drug Research Leiden University Medical Center Johan den Dunnen Leiden Genome Technology Center Leiden University Medical Center
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