Leiden Muscular Dystrophy pages©

Antibodies used to detect dystrophin

(last modified May 1, 2001)


The antibodies listed below are a collection of all those we have found in literature. When you notice that we have missed antibodies, when you detect mistakes or when you can give additional information, please please tell us. The list was collected with special thanks to Poala van de Bent in our laboratory.

Dystrophin antibody links:


Legend:
Antibody: name of antibody. Epitope: epitope of the antibody (usually the sequence used to raise the antibody), raised against a fp = fusion protein or syn = synthetic peptide; aa = amino acid. Type: type of antibody M = monoclonal or P = polyclonal. Species: antibody was raised in mouse, rabbit or unknown. IG-subtype: IG-subtype of the antibody (when known). Coupe: signal on tissue sections: + = clear signal, -  = no signal (chicken, human, mouse, rat, rabbit). Western: signal on Western blots; + = clear signal, - = no signal. Cross react: known cross reactivity. Supplier: supplier (when known).

Recently, Morris et al. published a paper entitled "An epitope structure for the C-terminal domain of dystrophin and utrophin", describing a detailed analysis of the MANDRA1-19 antibodies.

Antibody Epitope Type Species IG-
subtype
Coupe Western Cross react Reference Supplier
  aa 3675-3684 P     IF     Nicholson  
6C5
(= DY8 = Dys2)
aa 3668-3684 M mouse   + +   Ervasti Nova-Castra Laboratories Ltd
6D3
(= DY4)
aa 433-1233 M mouse G2a   hu+mo+rb+rat+   Nicolson
462B Dp71: -ex78 last 31 aa             Austin
1377 central rod         +   Burrow et al.'91
1461 aa 3668-3684       +   Bulman
9219 actin binding region         +   Bulman
Ab1 aa 12-29 P rabbit   + +   Winnard
Ab2 aa 235-252 P rabbit   +     Winnard
Ab3 aa 256-273 P rabbit   +     Winnard
Ab4 aa 2490-2507 > P rabbit   +     Winnard
CyDys3 aa 3357-3360 > P           (Fabbrizio et al. '93)
DY4
(= 6D3)
aa 433-1233 M mouse G2a   hu+mo+rb+rat+   Nicolson
DY8
(= 6C5 = Dys2)
aa 3668-3684 M mouse   + +   Ervasti Nova-Castra Laboratories Ltd
Dys1 aa 1181-1388 M mouse         Klein Nova-Castra Laboratories Ltd
Dys2
(= DY8 = 6C5)
aa 3668-3684 M mouse   + +   Ervasti Nova-Castra Laboratories Ltd
DYS3 aa 321-494 M mouse           Nova-Castra Laboratories Ltd.
MANDRA1 aa 3200-3684 M mouse G1 ++ mo++ fish++   Morris, Nguyen
MANDRA2 aa 3200-3684 M mouse G1 ++ mo++ fish+   Morris, Nguyen
MANDRA3 aa 3200-3684 M mouse G1 ++ mo+ fish+   Morris, Nguyen
MANDRA4 aa 3200-3684 M mouse G1 weak + mo+ fish+ mouse utrophin Morris, Nguyen
MANDRA5 aa 3200-3684 M mouse G2a + mo++ fish+   Morris, Nguyen
MANDRA6 aa 3200-3684 M mouse G1 +++ mo++ fish++   Morris, Nguyen
MANDRA7 aa 3200-3684 M mouse G1 ++ mo+ fish+   Morris, Nguyen
MANDRA8 aa 3200-3684 M mouse G1 weak + mo+ fish-   Morris, Nguyen
MANDRA9 aa 3200-3684 M mouse G1 ++ mo+ fish-   Morris, Nguyen
MANDRA10 aa 3200-3684 M mouse G1 weak + mo weak+ fish-   Morris, Nguyen
MANDRA11 aa 3200-3684 M mouse G1 weak + mo++ fish+   Morris, Nguyen
MANDRA12 aa 3200-3684 M mouse M weak + mo++ fish++   Morris, Nguyen
MANDRA13 aa 3200-3684 M mouse M weak + mo++ fish++   Morris, Nguyen
MANDRA14 aa 3200-3684 M mouse G1 weak + mo+   Morris, Nguyen
MANDRA15 aa 3200-3684 M mouse G2b weak + mo+ fish+ 200kDa Morris, Nguyen
MANDRA16 aa 3200-3684 M mouse G1 ++ mo++ fish++ mouse utrophin Morris, Nguyen
MANDRA17 aa 3200-3684 M mouse G1 ++ mo++ fish+ mouse utrophin Morris, Nguyen
MANDRA18 aa 3200-3684 M mouse G1 ++ mo++ fish++ mouse utrophin Morris, Nguyen
MANDRA19 aa 3200-3684 M mouse G1 + mo++ fish++ mouse utrophin Morris, Nguyen
MANDYS1 spectrin repeat rod domain M mouse         Nguyen
MANDYS2     mouse
MANDYS3     mouse
MANDYS101 aa 1749-2248 M mouse G2b + hu- mo- fish-   Nguyen
MANDYS102 aa 1749-2248 M mouse G2a + hu+ mo- fish-   Nguyen
MANDYS103 aa 1749-2248 M mouse G2a + hu+ mo- fish-   Nguyen
MANDYS104 aa 1749-2248 M mouse G2a + hu+ mo- fish-   Nguyen
MANDYS105 aa 1749-2248 M mouse G1 + (mo+,ch+) hu- mo- fish-   Nguyen
MANDYS106 aa 1749-2248 M mouse G2a + hu+ mo- fish-   Nguyen
MANDYS107 aa 1749-2248 M mouse G2b + hu- mo- fish-   Nguyen
MANDYS108 aa 1749-2248 M mouse G2a + hu+ mo- fish-   Nguyen
MANDYS109 aa 1749-2248 M mouse G1 + hu- mo- fish-   Nguyen
MANDYS110 aa 1749-2248 M mouse G1 + (mo+) hu- mo- fish-   Nguyen
MANDYS111 aa 1749-2248 M mouse G2a - hu- mo- fish- Fibers Nguyen
MANDYS121 aa 1749-2248 M mouse G1 - hu- mo+ fish+   Nguyen
MANDYS122 aa 1749-2248 M mouse G1 - hu- mo+ fish-   Nguyen
MANDYS123 aa 1749-2248 M mouse G1 - hu- mo+ fish-   Nguyen
MANDYS124 aa 1749-2248 M mouse G1 - hu- mo+ fish-   Nguyen
MANDYS125 aa 1749-2248 M mouse G2b - hu- mo+ fish-   Nguyen
MANDYS126 aa 1749-2248 M mouse G1 - hu- mo+ fish-   Nguyen
MANDYS127 aa 1749-2248 M mouse G1 - hu- mo+ fish-   Nguyen
MANDYS128 aa 1749-2248 M mouse G1 - hu- mo+ fish-   Nguyen
MANDYS129 aa 1749-2248 M mouse G1 - hu- mo+ fish-   Nguyen
MANDYS130 aa 1749-2248 M mouse G1 - hu- mo+ fish-   Nguyen
MANDYS131 aa 1749-2248 M mouse G1 - hu- mo+ fish-   Nguyen
MANDYS141 aa 1749-2248 M mouse G2b - hu- mo+ fish+ Actin Nguyen
MANDYS142 aa 1749-2248 M mouse G2a - hu- mo+ fish+ Actin Nguyen
MANDYS143 aa 1749-2248 M mouse G2b + hu- mo- Fast fibres Nguyen
NH2-Dys aa 1-15 P     +     Ervasti
P2O aa 1749-2248 P rabbit         Ginjaar


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