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The DMD cDNA in relation to the gene

(last modified March, 2010)

Examples of cDNA-hybridizations of HindIII and BglII can be found elsewheres. Pictures for TaqI have been published by Walker (1992).

Exon number Exon size (bp) Intron size (bp) 5' cDNA position Splice after RE-site HindIII- fragment BglII- fragment EcoRI- fragment PstI- fragment TaqI- fragment BamHI- fragment cDNA probe
Dp427c 151 319,091   -  1   6,213 8,219 4,537 7,247   41,938 -
Dp427m 275 191,081 (-244) 1 Bg-177 3,147 - Hd1 1,784+
7,136		 5,835 13,253   25,621 cDNA9-7
Dp427p 282 107.968   - 1               -
2 62 170,318 32 0   3,306 - Hd2 5,810 2,527 10,204   64,735 cDNA9-7
3 93 4,867 94 0   4,251 - Hd3 2,990 4,297 4,682   4.399 cDNA9-7
4 78 21,395 187 0 B199 8,351 - Hd4 12,869 2,077 2,809   1,139
(+33,940)		 cDNA9-7
5 93 6,654 265 0   2,985 - Hd5 7,019 10,117 4,432   33,940 cDNA9-7
6 173 6,856 358 2   7,753 - Hd6 3,671 12,130   cDNA9-7
7 119 110,199 531 1   4,571 - Hd7 1,174 4,074   15,159 cDNA9-7
8 182 1,113 650 0   7,214 / 8.3 - Hd8 6,271 / 2.92 2,015 4,936 /
8.52		   38,336 cDNA9-7
9 129 52,717 832 0   0,696 10,433   cDNA9-7
10 189 650 961 0   10,438 - Hd9 4,802 5,995 9,381/63   28,683 cDNA9-7
11 182 29,678 1150 2     cDNA9-7/cDNA30-2
12 151 18,426 1332 0   4,052 - Hd10 17,518 10,876 8,448/154   14,167 cDNA30-2
13 120 21,910 1483 0 Bg1494 6,364 - Hd11 (4,364+)
2,603		 3,831 12,693   4,123 cDNA30-2
14 102 107 1603 0   2,704 - Hd12 5,707 1,347 3,147   46,027 cDNA30-2
15 108 7,648 1705 0   4,825   cDNA30-2
16 180 20,367 1813 0 Bg1875 5,930 / 4,981 - Hd13
(c.1992+1801
C>G)		 3,214+
3,978		 3,559 3,065   cDNA30-2
17 176 27,027 1993 2   1,713 - Hd14 8,631 7,189 12,966   2,306 cDNA30-2
18 124 16,165 2169 0   11,040 - Hd15 15,952 11,079 5,292   13,311 cDNA30-2
19 88 10,236 2293 1   3,070 - Hd16 10,208 7,148 7,712   11,867 cDNA30-2
20 242 6,177 2381 0 E2440 7,301 - Hd17 (7,796) / 33,108 (c.2645G>A) (1,695+)
7,496		 13,109   8,069 cDNA30-2/cDNA30-1
21 181 12,609 2623

1

 2643Bg* 11,096 - Hd18 (7,796+)25,312 / 33,108 (c.2645G>A)

7,496

   29,273 cDNA30-1
22 146 3,453 2804 0   15,799 - Hd19 25,312 / 33,108 4,223 7,475
23 213 3,798 2950 0   8,644
24 114 991 3163 0   1,722 / 2,764
(c.3277-345
C>G)
25 156 8,606 3277 0   1,042 / 2,764
(c.3277-345
C>G)
26 171 6,023 3433 0 Bg3492 5,3891 - Hd20a (2,974+)
930		 8,184 12,793   11,456 cDNA30-1
27 183 7,141 3604 0   5,2491 - Hd20b 3,441   cDNA30-1
28 135 2,789 3787 0   10,597 - Hd21 4,871 7,426 14,095   17,690 cDNA30-1
29 150 26,327 3922 0   4,049   cDNA30-1
Dp260 95 248   - 0               -
30 162 21,570 4072 0   4,658 - Hd22 2,493 8,245 8,975   12,957 cDNA30-1
31 111 396 4234 0   14,763 - Hd23 12,281 5,457 8,143   17,816 cDNA30-1/cDNA47-4
32 174 3,035 4345 0   10,522   cDNA47-4
33 156 5,629 4519 0     cDNA47-4
34 171 15,310 4675 0   1,811 - Hd24 4,041 2,888   cDNA47-4
35 180 309 4846 0   358 - Hd25 3,018 8,725 2,712   14,091 cDNA47-4
36 129 1,623 5026 0   1,340 - Hd26   cDNA47-4
37 171 14,259 5155 0 5232S* (c.5234G>A),
P5244		 1,491 - Hd27 2,712
(+346)		   cDNA47-4
38 123 2,325 5326 0   5,797 - Hd28 21,977 10,930 10,962 / 144   14,088 cDNA47-4
39 138 2,656 5449 0     cDNA47-4
40 153 851 5587 0   6,140 - Hd29 2,593   34,123 cDNA47-4
41 183 31,823 5740 0     cDNA47-4
42 195 22,380 5923 0 Bg6087 4,203 - Hd30 6,690 / 2,213 (c.5923
-2047C>G)
(+1,039)		 2,297 8,518   22,999 cDNA47-4
43 173 70,465 6118 2   10,.810 - Hd31 6,153 5,546 12,654   19,919 cDNA47-4
44 148 248,401 6291 0 Bg6301 4,025 - Hd32 (5.076+)
3,281		 38,221 4,764 2,938  14,927 cDNA47-4
Dp140 >109 186.859   - -                
45 176 36,111 6439 2   470 - Hd33 2,731 4,275 3,525 1,898 923 cDNA47-4
46 148 2,334 6615 0 H6745 1,463 - Hd34
(+9,920)		 3,160 11,258 759 1,644 98,745 cDNA47-4
47 150 54,222 6763 0 E6794 9,920 - Hd35 3,391 (11,258+)
19,398		 5,282 3,859 (cDNA47-4)/cDNA44-1
48 186 38,368 6913 0 H6985 1,243+
3,906
- Hd36/37		 10,347 11,980 4,994 3,319 3,485 cDNA44-1
49 102 16,634 7099 0   1,614 - Hd38 7,286 7,140 6,529 4,797 22,851 cDNA44-1
50 109 45,782 7201 1 Bg7212 3,745 - Hd39 (3,576+)
2,547		 21,762 821 4,868 cDNA44-1
51 233 44,211 7310 0 3,153 - Hd40 1,201 7,639 3,563 / 10,650
(c.7310-2022
G>T)		 7,671
(6.8/5.76)		 26,278 cDNA44-1
52 118 50,044 7543 1 T7,656 7,153 - Hd41 3,447 5,852 12,250 2,067
(+1,387)		 22,181 cDNA44-1
53 212 21,230 7661 0 E7667,
H7804		 7,446
(+1,045)
- Hd42/43		 13,384 (1,125+)
5,040		 6,695 4,014 10,167 cDNA63-1/1
54 155 30,127 7873 2 P7955 8,045 - Hd44 9,140 3,716  4,636
(+8,026)		 2,849 13,989 cDNA63-1/1
55 190 120,219 8028 0   2,304 - Hd45 7,642 22,583 16,091 3,956 8,803 cDNA63-1/1
Dp116 91 755   - 0     8,898         -
56 173 10,336 8218 2   8,735 - Hd46 2,882 19,817 4,877 32,106 cDNA63-1/1
57 157 17,684 8391 0 P8440 1,040 - Hd47 4,011 15,927 (19,817+)
6,356		 9,543 cDNA63-1/1
58 121 608 8548 1   6,002 - Hd48 5,894 2,256 4,303 1,784
(+15...)		 cDNA63-1/1
59 269 33,478 8669 0 B8872 2,461 32,106
(+1,902)		 cDNA63-1/1+2
60 147 95,846 8938 0 H9073,
P9079		 3,612
(+2,806) - Hd49		 4,266 4,386 2,399   17,311 cDNA63-1/2
61 79 24,897 9085 1   6,823 - Hd50 9,498 4,013 5,297   29,262 cDNA63-1/2
62 61 62,581 9164 2   2,902 2,640 5,478 15,162   26,741 cDNA63-1/2
Dp71/Dp40 176 5,793   - 2   1.8            
63 62 37,833 9225 1   12,330 1,209 10,306 7,182   8,329 cDNA63-1/2
64 75 13,347 9287 1 P9343 2,451 3,130 3,169 916   14,549 cDNA63-1/2
65 202 2,830 9362 2 P9442 2,637 1,335 1,429 4,501+
3,693		   5,766 cDNA63-1/2
66 86 2,463 9564 1 B9578 2,072 3,900 7,727 3,693   (5,766+)
6,590		 cDNA63-1/3
67 158 21,056 9650 0   1,429 925   6,590 cDNA63-1/3
68 167 2,256 9808 2 P9908 6,753 23,829 11,230 7,601
(+2,501)		   23,439 cDNA63-1/3
69 112 1,564 9975 0   2,501   cDNA63-1/3
70 137 698 10087 2   1,505 9,864 4,263   cDNA63-1/3
71 39 4,327 10224 2     cDNA63-1/3
72 66 1,125 10263 2   2,044 7,741

6,266

   50,628 cDNA63-1/3
73 66 2,746 10329 2   1,886   cDNA63-1/3
74 159 21,924 10395 2 Bg10478,
Bg10533		 2,531 9,864
+55		       cDNA63-1/3
75 244 860 10554 0   9,873 3,875 4,899 19,489   cDNA63-1/4
76 124 12,096 10798 1   1,225   cDNA63-1/4+HcE
77 93 7,428 10922 1 Bg10929 2,034 (9,119+)
5,691		 9,618 2,991   cDNA63-1/HcE
78 32 4,711 11015 0   3,378 17,599 5,355 14,248   14,297 cDNA63-1/HcE
79 2703

-

 11047   H12764 6,071+ 8,158 10,125   cDNA63-1/HcE+e

Legend:
Data have been derived from the Reference Sequence of the DMD-gene. Exon number: numbering of exons is according to Roberts et al. (1993). Exon size (bp): size of exon in base pairs. Intron size (bp): size of intron in base pairs. 5' cDNA position: first base of the exon (according to the DMD coding DNA Reference Sequence, counting the A of the ATG translation initiation codon as nucleotide 1). Splice after: splicing occurs in between of two coding triplets (=0), after the first (1) or after the second (2) base of a triplet. RE-site: restriction enzyme recognition sequence in the exon with the most 5' nucleotide at the position indicated. B = BamHI, Bg = BglII, E = EcoRI, H = HindIII, P = PstI, S = SacII (*=polymorphic site). HindIII fragment, BglII, EcoRI, PstI: length of restriction fragment for the respective enzyme containing the exon. Sizes are given in base pairs, / = polymorphic variants, (n) = fragment hardly or not detectable. cDNA probe: cDNA probe which can be used to detect the exon on a Southern blot.
1polymorphic HindIII-fragment 5.2 or 5.3 kb (unknown if exon 26 or 27, den Dunnen [1989], Hu [1990], Lindor [1993] 4/19). 2polymorphic BglII 6.5/2.9 kb and 7.5/8.3 HindIII-fragments and possibly 4.8/8.5 kb PstI-fragment (exons 8 and 9, Prior [1992] 0.33 African-Americans (0.005 Caucasian), Lindor [1993] 10/21 Afriacan-Americans). 3polymorphic 9.4/6 kb PstI-fragment exons 10+11 (Lindor [1993] 1/19 Afriacan-Americans). 4polymorphic 8.5/15 kb PstI-fragment exon 12 (Lindor [1993] 1/19 Afriacan-Americans). 5polymorphic 11.8/14 kb PstI-fragment exons 38+39 (Lindor [1993] 6/19 African-Americans). 6polymorphic 6.8/5.7 kb TaqI-fragment exon 51 (Walker [1992] 7/125 (USA).

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