Leiden Muscular Dystrophy pages

Antibodies against proteins of the Dystrophin Glycoprotein Complex

(last modified February 4, 2006)


Contents



Introduction


The antibodies listed below are a collection of those we have found in literature. Often, many details are lacking in these publications so we are not able to provide them. When you notice that we have missed antibodies, when you detect mistakes or when you can give additional information, please tell us immediately.


Dystrophin antibody links



Antibody tables


alpha-/beta-Dystroglycan  (DAG1)

Antibody Epitope Type M/P Raised in IG-subtype On coupe On Western Cross react. Reference Supplier
IIH6     M mouse     + beta- Ervasti
FP-D aa 062-438 fp (GEX-) P guinea pig   + + alpha-/beta- Ibraghimov-Beskrovnaya
FP-B aa 367-863 fp (GEX-) P guinea pig     + alpha-/beta- Ibraghimov-Beskrovnaya
FP-A aa 665-856 fp (GEX-) P guinea pig   + + alpha- Ibraghimov-Beskrovnaya
FP-C aa 857-895 fp (GEX-) P guinea pig     + alpha- Ibraghimov-Beskrovnaya
43 C-term aa 880-894 synthetic P rabbit     + alpha- Ibraghimov-Beskrovnaya
PA3b           +     Yoshida
43DAG/8D5     M           Cullen/Anderson
VIA41     M       + beta- Ibraghimov-Beskrovnaya

Legend:
Antibody: name of antibody. Epitope: epitope of the antibody (usually the sequence used to raise the antibody). Type: type of epitope used to raise the antibody fp = fusion protein, syn = synthetic peptide. M/P antibody is M = monoclonal, P = polyclonal. Raised in: animal in which the antibody was raised. IG-subtype: IG-subtype of the antibody. On coupe: signal on tissue sections: + = clear signal, - = no signal (ch=chicken, hu=human, mo=mouse, rat=rat, rb=rabbit). On Western: signal on Western blots: + = clear signal, - = no signal (ch=chicken, hu=human, mo=mouse, rat=rat, rb=rabbit). Cross react: observerd cross reactivity wit. Reference: publication providing details regarding the antibody. Supplier: supplier (if known).


alpha-Sarcoglycan  (SGCA)

Antibody Epitope Type M/P Raised in IG-subtype On coupe On Western Cross react. Reference Supplier
50DAG (Ad1/20A6) aa 217-289 (rabbit) fp (GST-) M mouse   + +   McNally, LVB Anderson Novacastra Laboratories Ltd.
Ad1/20A6 (50DAG) aa 217-289 (rabbit) fp (GST-) M mouse   + +   McNally, LVB Anderson Novacastra Laboratories Ltd.
Ad1/20A5z     M mouse   +     Nigro
IVD31 fpG and fpH fp (GST-) M mouse   +     Matsumara
Anti-FP-G aa 217-289 (rabbit) fp (GST-)   sheep     +   Roberds
Anti-FP-H aa 312-387 (rabbit) fp (GST-)   sheep     +   Roberds
C-term aa 376-387 (rabbit) synthetic   rabbit     +   Roberds

Legend:
Antibody: name of antibody. Epitope: epitope of the antibody (usually the sequence used to raise the antibody). Type: type of epitope used to raise the antibody fp = fusion protein, syn = synthetic peptide. M/P antibody is M = monoclonal, P = polyclonal. Raised in: animal in which the antibody was raised. IG-subtype: IG-subtype of the antibody. On coupe: signal on tissue sections: + = clear signal, - = no signal (ch=chicken, hu=human, mo=mouse, rat=rat, rb=rabbit). On Western: signal on Western blots: + = clear signal, - = no signal (ch=chicken, hu=human, mo=mouse, rat=rat, rb=rabbit). Cross react: observerd cross reactivity wit. Reference: publication providing details regarding the antibody. Supplier: supplier (if known).


-Sarcoglycan  (SGCB)

Antibody Epitope Type M/P Raised in IG-subtype On coupe On Western Cross react. Reference Supplier
? full cDNA fp (GST-) P rabbit   + +   Bonnemann
?     P rabbit         Nigro
43DAG1 (8D5)     M mouse   + +     Novacastra Laboratories Ltd.
8D5 (43DAG1)     M mouse   + +     Novacastra Laboratories Ltd.
? aa 161-174 synthetic       + +   Bonnemann
anti-FP-I aa 89-152 fp (GST-) P rabbit   + +   Lim
anti-PF-II aa 42-52 synthetic P sheep   +     Lim

Legend:
Antibody: name of antibody. Epitope: epitope of the antibody (usually the sequence used to raise the antibody). Type: type of epitope used to raise the antibody fp = fusion protein, syn = synthetic peptide. M/P antibody is M = monoclonal, P = polyclonal. Raised in: animal in which the antibody was raised. IG-subtype: IG-subtype of the antibody. On coupe: signal on tissue sections: + = clear signal, - = no signal (ch=chicken, hu=human, mo=mouse, rat=rat, rb=rabbit). On Western: signal on Western blots: + = clear signal, - = no signal (ch=chicken, hu=human, mo=mouse, rat=rat, rb=rabbit). Cross react: observerd cross reactivity wit. Reference: publication providing details regarding the antibody. Supplier: supplier (if known).


gamma-Sarcoglycan  (SGCG)

Antibody Epitope Type M/P Raised in IG-subtype On coupe On Western Cross react. Reference Supplier
? aa 3-291 fp (GST-) P rabbit   + +   McNally Novacastra Laboratories Ltd.
35DAG (21B5)     M     +     Nigro, Sewry
? aa 60-291 fp (GST-) P       +   Nigro
MA4-2           +     Yamamoto 1994, J.Biochem.115">162-167

Legend:
Antibody: name of antibody. Epitope: epitope of the antibody (usually the sequence used to raise the antibody). Type: type of epitope used to raise the antibody fp = fusion protein, syn = synthetic peptide. M/P antibody is M = monoclonal, P = polyclonal. Raised in: animal in which the antibody was raised. IG-subtype: IG-subtype of the antibody. On coupe: signal on tissue sections: + = clear signal, - = no signal (ch=chicken, hu=human, mo=mouse, rat=rat, rb=rabbit). On Western: signal on Western blots: + = clear signal, - = no signal (ch=chicken, hu=human, mo=mouse, rat=rat, rb=rabbit). Cross react: observerd cross reactivity wit. Reference: publication providing details regarding the antibody. Supplier: supplier (if known).


delta-Sarcoglycan (SGCD)

Antibody Epitope Type M/P Raised in IG-subtype On coupe On Western Cross react. Reference Supplier
deltaSG aa 84-290 fp (GST-) P rabbit   + +   Nigro

Legend:
Antibody: name of antibody. Epitope: epitope of the antibody (usually the sequence used to raise the antibody). Type: type of epitope used to raise the antibody fp = fusion protein, syn = synthetic peptide. M/P antibody is M = monoclonal, P = polyclonal. Raised in: animal in which the antibody was raised. IG-subtype: IG-subtype of the antibody. On coupe: signal on tissue sections: + = clear signal, - = no signal (ch=chicken, hu=human, mo=mouse, rat=rat, rb=rabbit). On Western: signal on Western blots: + = clear signal, - = no signal (ch=chicken, hu=human, mo=mouse, rat=rat, rb=rabbit). Cross react: observerd cross reactivity wit. Reference: publication providing details regarding the antibody. Supplier: supplier (if known).


Dystrobrevin

Antibody Epitope Type M/P Raised in IG-subtype On coupe On Western Cross react. Reference Supplier
24H3 Torpedo 87kD   M           Carr J.Cell Biol. 109;1753-1764
PEP89 aa 13-29 syn P rabbit     + sk.muscle myosin Sadoulet-Puccio

Legend:
Antibody: name of antibody. Epitope: epitope of the antibody (usually the sequence used to raise the antibody). Type: type of epitope used to raise the antibody fp = fusion protein, syn = synthetic peptide. M/P antibody is M = monoclonal, P = polyclonal. Raised in: animal in which the antibody was raised. IG-subtype: IG-subtype of the antibody. On coupe: signal on tissue sections: + = clear signal, - = no signal (ch=chicken, hu=human, mo=mouse, rat=rat, rb=rabbit). On Western: signal on Western blots: + = clear signal, - = no signal (ch=chicken, hu=human, mo=mouse, rat=rat, rb=rabbit). Cross react: observerd cross reactivity wit. Reference: publication providing details regarding the antibody. Supplier: supplier (if known).


FKRP  (fukutin-related protein)

Antibody Epitope Type M/P Raised in IG-subtype On coupe On Western Cross react. Reference Supplier
                 
FKRP-FP
(5643)
aa 36-end fp P rabbit   + +   Esapa et al. (2002)

FCMD  (Fukuyama type congenital muscular dystrophy, fukutin)

Antibody Epitope Type M/P Raised in IG-subtype On coupe On Western Cross react. Reference Supplier
                 
FK-CT last 60 aa (murine) fp P rabbit   + +   Esapa et al. (2002)


Utrophin

Recently, Morris et al. published a paper entitled "An epitope structure for the C-terminal domain of dystrophin and utrophin", describing a detailed analysis of the MANCHO1-19 antibodies.

Protein Antibody Epitope Type M/P Raised in IG-subtype On coupe On Western Cross react. Reference Supplier
Utrophin
(UTRN)
MANCHO 1 last 329 aa               Morris, Nguyen  
  MANCHO 2 last 329 aa               Morris, Nguyen  
  MANCHO 3 last 329 aa               Morris, Nguyen  
  MANCHO 4 last 329 aa               Morris, Nguyen  
  MANCHO 5 last 329 aa               Morris, Nguyen  
  MANCHO 6 last 329 aa               Morris, Nguyen  
  MANCHO 7 last 329 aa               Morris, Nguyen  
  MANCHO 8 last 329 aa               Morris, Nguyen  
  MANCHO 9 last 329 aa               Morris, Nguyen  
  MANCHO 10 last 329 aa               Morris, Nguyen  
  MANCHO 11 last 329 aa               Morris, Nguyen  
  MANCHO 12 last 329 aa               Morris, Nguyen  
  MANCHO 13 last 329 aa               Morris, Nguyen  
  MANCHO 14 last 329 aa               Morris, Nguyen  
  MANCHO 15 last 329 aa               Morris, Nguyen  
  MANCHO 16 last 329 aa               Morris, Nguyen  
  MANCHO 17 last 329 aa               Morris, Nguyen  
  MANCHO 18 last 329 aa               Morris, Nguyen  
  MANCHO 19 last 329 aa               Morris, Nguyen  
Titin
(TTN)
  aa 1-195 (Z1- Z2) fp P rabbit     +   Gregorio (1998)  
    aa 560-650
(rabbit, Zr5-Z6)
fp P rabbit     +   Gregorio (1998)  
  N-terminal Ig29 repeat fp P rabbit   + +   Bang (2001)  

Legend:
Antibody: name of antibody. Epitope: epitope of the antibody (usually the sequence used to raise the antibody). Type: type of epitope used to raise the antibody fp = fusion protein, syn = synthetic peptide. M/P antibody is M = monoclonal, P = polyclonal. Raised in: animal in which the antibody was raised. IG-subtype: IG-subtype of the antibody. On coupe: signal on tissue sections: + = clear signal, - = no signal (ch=chicken, hu=human, mo=mouse, rat=rat, rb=rabbit). On Western: signal on Western blots: + = clear signal, - = no signal (ch=chicken, hu=human, mo=mouse, rat=rat, rb=rabbit). Cross react: observerd cross reactivity wit. Reference: publication providing details regarding the antibody. Supplier: supplier (if known).



| Top of page | LMDp homepage |
| dystrophin antibodies |
| Remarks / informationCopyright, liability |